Ukukhulisa Isithiyo Esisebenza Ngegazi

Ngabasebenzi Brain & Behaeve

Abaphenyi abaholwa ngokubambisana ngu-2016 BBRF Umphenyi Omncane u-Ethan Lippmann, Ph.D., babike ukuthi baphumelele “ekwakheni” izicubu zemithambo ezisebenza njengolwelwesi olubalulekile lokuvikela ubuchopho, olubizwa ngokuthi yisithiyo segazi nobuchopho. Isithiyo sisebenza njengesefa ekhethiwe, sigcina ama-molecule amakhulu afaka amabhaktheriya ngaphandle kobuchopho nomgogodla, kepha avumela i-oxygen, i-glucose, nezinye izinto ezibalulekile ukuthi zingene.

Umsebenzi, owenziwe eVanderbilt University futhi washicilelwa ngoFebhuwari 14, 2019 kuStem Cell Reports, kufanele usize ukusheshisa ukuhunyushwa kwemibono yesayensi ocwaningweni lobuchopho.

Ngenkathi amasiko obuchopho obungamabili-amaseli ekhulisiwe esikhathini esedlule, lesi yisikhathi sokuqala imodeli enezinhlangothi ezintathu esebenza njengesithiyo segazi lobuchopho bomuntu. Imodeli ikhula kusuka kumaseli athathwe kusampula yomuntu akhuthazwa ukuthi akhe kabusha njengohlobo lweseli olukhethekile oluyisisekelo sesithiyo segazi nobuchopho. Ngemuva kwalokho ahlanganiswe ku-matrix enezinhlangothi ezintathu esebenza njengesafa.

Le nqubo yokwenza amaseli kabusha, eyaphayona ocwaningweni lobuchopho ngabahlinzeki be-BBRF nabanye kule minyaka eyishumi edlule, ibizwa nge-iPSC, emele ubuchwepheshe “obunamandla be-stem cell”. Inokusetshenziswa okuningi emithini yezokwelapha, ikakhulukazi ekwakhiweni kwezinhlobo ezahlukahlukene ze- "organoids" -eziphilayo, amasiko anezinhlangothi ezintathu zamaseli ahlanganisiwe ukwakhiwa kabusha njengezinhlobo zamaseli ezithize ezithweni ezahlukahlukene zomzimba. Enye indlela ethembisayo eya phambili ekuhlolweni kwezidakamizwa nasekucwaningeni ngezifo ilele ekwakheni amamodeli we-organoid wezitho zomuntu, ukuthola ukusebenza kwemithi namandla.

Ngenkathi abacwaningi bezame ama-organoid obuchopho obudlangile, indlela entsha yokwakha kabusha izakhiwo ezenza indima yesithiyo segazi lobuchopho bomuntu, uma ifakwa kuma-organoids obuchopho, izoletha isayensi isinyathelo esikhulu eduze nokwakha "ubuchopho esitsheni" ngokuthembeka phindaphinda kokubili ukwakheka nokusebenza kobuchopho bomuntu uqobo, noma izingxenye zazo.

Ukuphinda kabili isithiyo se-endothelial ku-organoids yobuchopho kubalulekile, ngoba ubuchopho kufanele buvikelwe ezintweni ezisegazini.

Isithiyo segazi nobuchopho siqala "ukuvuza" ezifweni ezithile, kufaka phakathi izifo ezithile zemizwa kubandakanya i-ALS nesifo sokuwa. Kubuye kuvunyelwe kakhulu lapho ukuvuvukala emzimbeni kufinyelela emazingeni aphezulu. Lokhu kungaba yindlela eyodwa lapho ama-molecule wokuvuvukala angena ebuchosheni nasekuphazamiseni umsebenzi ojwayelekile, ngokwesibonelo ku-multiple sclerosis.